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24056

Supelco

SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

Synonym(s):

SP-2560, 100M.20UM.25MM

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About This Item

UNSPSC Code:
41115710
eCl@ss:
32119290
Pricing and availability is not currently available.

material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

313

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

100 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
293137-04
293137-03
293136-07
293136-06
293136-05

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Antonio Natalello et al.
Journal of agricultural and food chemistry, 67(16), 4472-4482 (2019-04-02)
This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with
B Schettino et al.
Journal of dairy science, 100(8), 6256-6265 (2017-06-12)
Chia seed (Salvia hispanica L.) is the greatest known plant source of n-3 α-linolenic acid. The present study evaluated the effects of 3 inclusion levels of chia seed [zero (control); low, 2.7% (CLow); and high, 5.5% (CHigh)] in diets of
Evaluation of the CP-Sil 88 and SP-2560 GC columns used in the recently approved AOCS official method Ce 1h-05: Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC method.
Ratnayake WMN
Journal of the American Oil Chemists' Society, 83 (6), 475-488 (2006)
Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry.
Alexander LR
Journal of Chromatography. B, Biomedical Applications, 342, 1-12 (1985)
Adriana Campione et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-05)
The hazelnut skin is waste biomass rich in bioactive compounds that may affect lipid rumen metabolism, ruminant performance, and products' quality. Therefore, we investigated the effect of dietary hazelnut skin on milk production and composition and on rumen fatty acids
View All Related Papers

Protocols

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

GC Analysis of a 37-Component FAME Mix on SP™-2560

A protocol for GC Analysis of a 37-Component FAME Mix on SP™-2560.

FAME Standard for Optimizing GC System Performance

AOCS and AOAC methods for determining the trans fatty acid composition of foods by gas chromatography require the use of a highly polar cyanosilicone capillary column to provide the best peak resolution attainable of the numerous geometric (cis & trans) and positional isomers.

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Chromatograms

suitable for GCsuitable for GCsuitable for GC, application for SPEsuitable for GC

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the safety temperature for the GC instrument with SP®-2560 Capillary GC Column (P/N: 24056) to turn off without damaging the column?

    1 answer

    1. It is recommended for lowering the temperature of the GC to 35°C or below with gas flow before shutting the instrument off. If the column will not be in use for an extended period, it is recommended to remove it from the GC and place septa or caps on each end.

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