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Z763624

Breathe Easier sealing membrane for multiwell plates

pack of 100 ea

Synonym(s):

breathable sealing membrane, multiwell sealing film, plate sealers, plate sealing film, sealing film

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About This Item

UNSPSC Code:
41122410
NACRES:
NB.22
Pricing and availability is not currently available.

material

white Rayon plate (non-woven)
white wells

sterility

sterile

feature

piercable

packaging

pack of 100 ea

manufacturer/tradename

Diversified Biotech BERM-2000

size

96 wells

color

white

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General description

Gas-Permeable Sealing Membrane - A Fresh Breath for Cell Growth Technology!

Breathe-Easier sealing membrane offers an exceedingly high rate of gas exchange to allow maximum cell growth rate. When adhered to a microtiter plate, the 5.5 mil thick membrane insures a uniform rate of gas exchange for all wells, while sustaining a contaminant-free environment within the well. Conventional microtiter lids disrupt uniform gas exchange from well to well by favoring those wells along the perimeter of the plate.

With Breathe-Easier, sterility is achieved using gamma irradiation. Unlike ethylene oxide sterilization, this sterilization method assures that no cytotoxins are introduced into the membranes. Breathe Easier will not inhibit fibroblast cell growth or division, and the membrane is FDA approved for blood and cell contact in wond healing. The acrylic adhesive exhibits excellent bonding characteristics with all different plastic microtiter plates.

  • High Gas Permeability- Rapid and uniform transmission of oxygen and exchange of carbon dioxide to stimulate cell growth. Water vapor transmission across membrane is 4.2 kg/m2/24 hours.
  • Non-Cytotoxic and Sterile- Membranes are gamma irradiated to assure sterility. Membranes are free of cytotoxins.
  • Thermal & Chemical Stability- Resists a majority of caustic agents and organic solvents as well as exposure to temperatures from -20 C to +80 C.
  • Simple Specimen Retrieval- Samples can be removed from plates with Breathe-Easier in place since membranes can be pierced with pipette tips.
  • Practically Designed & User-Friendly- Breathe-Easier format is 6 x 3.5 in. rectangles that fit 96-well plates, bio-blocks and tissue culture plates. Paper release liner is scored for easy application of membranes to plates.

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Justin I Yoo et al.
Nature communications, 11(1), 5459-5459 (2020-10-31)
Biocontainment systems are needed to neutralize genetically modified organisms (GMOs) that pose ecological threats outside of controlled environments. In contrast, benign selection markers complement GMOs with reduced fitness. Benign selection agents serve as alternatives to antibiotics, which are costly and

Questions

1–4 of 4 Questions  

  1. Are the films resealable after taking sample or does a new film have to be used?

    1 answer

    1. This product has not been tested for resealing integrity. While it is likely that the films can be reapplied, a patent seal cannot be guaranteed. To ensure sterility, applying a new film would be recommended.

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  2. Does it prevent edge effect?

    1 answer

    1. Unfortunately, the edge effect has not been measured.

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  3. Whar are the dimensions of the membrane?

    1 answer

    1. As stated in the description section of the product page, the sealing membrane dimensions are 6 inches x 3.5 inches.

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  4. can it maintain sterility while allowing aerobic bacteria to have oxygen?

    1 answer

    1. These sealing membranes allow for gas exchange while preventing microbial contamination of cultures. Provided that the plate and contents are free of contaminants prior to sealing, the cultures will be protected during incubation.

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