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U2258

Sigma-Aldrich

Ubiquitin-7-amido-4-methylcoumarin

≥95% (HPLC), solution (supplied in DMSO)

Synonym(s):

Ubiquitin-AMC

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About This Item

MDL number:
MFCD02100846
UNSPSC Code:
12352204
NACRES:
NA.32
Pricing and availability is not currently available.

Product Name

Ubiquitin-7-amido-4-methylcoumarin, solution (supplied in DMSO)

Assay

≥95% (HPLC)

Quality Level

100

form

solution (supplied in DMSO)

shipped in

dry ice

storage temp.

−70°C

General description

Manufactured for Sigma by Boston Biochem. Inc.
Ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) is a fluorogenic Ub-base probe. It consists of AMC conjugated to the glycine residue of ubiquitin.[1] Ub-AMC is a fluorogenic substrate for ubiquitin C-terminal hydrolase-L3 (UCH-L3) as well as other UCH isozymes.[2]

Application

Ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) has been used as a fluorogenic substrate in deubiquitinase assay buffer.[3]

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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Certificates of Analysis (COA)

Lot/Batch Number
SLBV2250
SLBG7112
081M1175
100M0485
070M0544

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Efficient chemical synthesis for the analogue of ubiquitin-based probe Ub--AMC with native bioactivity
Xu L, et al.
Royal Society of Chemistry Advances, 6(53), 47926-47930 (2016)
Kazunori Hirayama et al.
Bioorganic & medicinal chemistry, 15(21), 6810-6818 (2007-09-01)
UCH-L3 (ubiquitin C-terminal hydrolase-L3) is a de-ubiquitinating enzyme that is a component of the ubiquitin-proteasome system and known to be involved in programmed cell death. A previous study of high-throughput drug screening identified an isatin derivative as a UCH-L3 inhibitor.
Sehyoun Yoon et al.
Neuron, 105(3), 506-521 (2019-12-10)
Variants in the ANK3 gene encoding ankyrin-G are associated with neurodevelopmental disorders, including intellectual disability, autism, schizophrenia, and bipolar disorder. However, no upstream regulators of ankyrin-G at synapses are known. Here, we show that ankyrin-G interacts with Usp9X, a neurodevelopmental-disorder-associated deubiquitinase (DUB).

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