This product has not been tested for use in Immunofluorescence. The end user would have to determine suitability. Regarding the questions of co-staining, primary antibodies of the same animal species and/or cross-reacting antibodies can be combined, provided that the antigens are localized in different cell types or in different areas of the same cell.
As there are additional questions that should be addressed it would be best to contact technical service. Please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.
SAB5200058
Monoclonal Anti-KCNA4 antibody produced in mouse
clone S13-31, 1 mg/mL, purified immunoglobulin
Synonym(s):
Anti-HBK4, Anti-HK1, Anti-HPCN2, Anti-HUKII, Anti-KCNA4, Anti-Kv1.4 K+ channel, S13-31
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
S13-31, monoclonal
form
buffered aqueous glycerol solution
mol wt
antigen predicted mol wt 98 kDa
species reactivity
mouse, human, rat
concentration
1 mg/mL
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Kcna4(25469)
Specificity
Immunogen
Features and Benefits
Physical form
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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can this mouse KV1.4 antibody work in IF? Can I co-stain mouse cells by using 2 primary antibodies from rabbit?
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