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SAB4700113

Sigma-Aldrich

Monoclonal Anti-CD19-PE antibody produced in mouse

clone LT19, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

phycoerythrin (R-PE) conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LT19, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD19(930)

General description

CD19 (cluster of differentiation 19) is a 95kDa protein localized on the surface of nearly all B lymphocytes and is considered to be the specific surface marker for B cells.This protein belongs to the immunoglobulin (Ig) superfamily with a cytoplasmic region of 240 amino acids and is involved in the regulation of B cell function.The protein is encoded by the 7.41kb cd19 gene, mapped on the short arm of human chromosome 16p11.2. There are certain localized regions of strong net negative charges with nine tyrosine residues in the cytoplasmic region of CD19. These regions might regulate the interaction of CD19 with the proto-oncogene Vav, phosphatidylinositol-3 kinase (PI-3 kinase) and the Lyn, Lck and Fyn protein tyrosine kinases.

Immunogen

Daudi human Burkitt lymphoma cell line

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Biochem/physiol Actions

CD19 (cluster of differentiation 19) expression is restricted to normal and malignant B-lineage cells. Therefore, it is considered to be a striking target for immunotherapies aimed at B-cell malignancies. CD19 helps B lymphocytes to initiate primary immune response even at low concentrations of antigen.The encoded protein plays a crucial role in initiating intrinsic B cell signaling thresholds by regulating both B cell receptor (BCR)-dependent and independent signaling. It also functions as a biomarker for B-cell development.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation.
Pezzutto A
Journal of Immunology, 138, 2793-2799 (1987)
CD19: a biomarker for B cell development, lymphoma diagnosis and therapy.
Wang K
Experimental Hematology & Oncology, 1 (2012)
The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity.
Fearon DT and Carter RH.
Annual Review of Immunology, 127-149 (1995)
The CD19-CD21 complex regulates signal transduction thresholds governing humoral immunity and autoimmunity.
Tedder TF
Immunity, 6, 107-118 (1997)
B-cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor-transduced T cells.
Kochenderfer JN
Blood, 119, 2709-2720 (2012)

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