SAB4200699

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal

clone TH-2, purified from hybridoma cell culture

• Synonym(s): TH, TYH
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TH-2, monoclonal
• form: buffered aqueous solution
• species reactivity: rabbit, human, sheep, guinea pig, rat, bovine, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.25-0.5 μg/mL using whole extracts of rat PC-12 cells.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TH(7054)

Description

General description

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TH-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat Tyrosine Hydroxylase. Tyrosine Hydroxylase (TH) also known as tyrosine 3- monooxygenase, is encoded by the gene mapped to human chromosome 11p15.5. It is a tetrameric enzyme composed of four subunits. Four TH Isoforms were identified in humans.

Immunogen

purified rat Tyrosine Hydroxylase

Application

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal has been used in various immunochemical techniques including immunoblotting, immunohistochemistry, immunofluorescence and immunoprecipitation.

Biochem/physiol Actions

Tyrosine Hydroxylase (TH) catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxyphenylalanine (L-dopa) in brain and adrenal medulla which is the initial and rate limiting step of the neurotransmitters and hormones catecholamines (dopamine, norepinephrine and epinephrine) biosynthesis. Therefore, TH plays a key role in the physiology of adrenergic neurons. Tyrosine Hydroxylase (TH) deficiency is associated with the development of wide variety of phenotypic spectrum such as several types of dystonia, early-onset of Parkinson′s, cerebral palsy or spastic paraplegia and both primary and secondary deficiencies of cerebrospinal fluid (CSF) neurotransmitter metabolites.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable