SAB3700182
Anti-Chicken IgG F(ab′)2-Peroxidase antibody produced in rabbit
affinity isolated antibody, lyophilized powder
Synonym(s):
HRP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
chicken
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Chicken IgG (immunoglobulin G) is also referred to as IgY. IgY is an antibody produced in birds. IgY is similar to mammalian IgG but differs in some characteristics from IgG. IgY does not bind with mammalian complements nor rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies. During oogenesis in chicken, IgY can pass across the follicular epithelium of the ovary and aggregate in the yolk. The heavy chain of IgY and mammalian IgG has a molecular weight of 68kDa and 50kDa, respectively. The structure of mammalian IgG is considered more stable than that of IgY. IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. Thus, IgY is beneficial when it is used as a biochemical/clinical reagent.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab′)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
Immunogen
Chicken IgG F(ab′)2 fragment
Physical properties
Antibody format: IgG
Physical form
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Reconstitution
Reconstitute with 1.0 mL deionized water (or equivalent).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Production and purification of Fab? fragments from chicken egg yolk immunoglobulin Y (IgY)
Akita EM and Nakai S
Journal of Immunological Methods, 162(2), 155-164 (1993)
Molecular stability of chicken and rabbit immunoglobulin G.
Shimizu M
Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274 (1992)
Immunoglobulins of the non-galliform birds: antibody expression and repertoire in the duck.
Lundqvist ML
Developmental and Comparative Immunology, 30(1-2), 93-100 (2006)
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