Skip to Content
Merck
All Photos(4)

Key Documents

SAB2702197

Sigma-Aldrich

Monoclonal Anti-GFP antibody produced in mouse

clone GT859, affinity isolated antibody

Synonym(s):

Mouse Monoclonal Anti-Green Fluorescent Protein

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT859, monoclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

1mg/mL

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1000-10000

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Green fluorescent protein (GFP) is a fluorescent protein. It is mainly isolated from the luminous organ of the jellyfish Aequorea victoria.

Immunogen

Full length GFP recombinant protein

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IHC-Fr: Assay-dependent dilution, IHC-P: Assay-dependent dilution, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-GFP antibody has been used in gait analysis.

Biochem/physiol Actions

Green fluorescent protein (GFP) is used as a marker for gene expression. It is also used as a tag to study about protein localization in several organisms. GFP can form the intrinsic chromophore without the help of cofactors or enzymatic components.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
41162

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zac1/GPR39 phosphorylating CaMK-II contributes to the distinct roles of Pax3 and Pax7 in myogenic progression
Yang Q, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 407-419 (2018)
FACS-optimized mutants of the green fluorescent protein (GFP)
Cormack B P, et al.
Gene, 173(1), 33-38 (1996)
Fluorescence-based biosensors: from concepts to applications
Progress in Molecular Biology and Translational Science, 113(1) (2012)
Yang Yang et al.
Nature neuroscience, 19(10), 1348-1355 (2016-09-07)
Neural circuits underlying auditory fear conditioning have been extensively studied. Here we identified a previously unexplored pathway from the lateral amygdala (LA) to the auditory cortex (ACx) and found that selective silencing of this pathway using chemo- and optogenetic approaches
Kai Lǚ et al.
Viruses, 14(5) (2022-05-29)
A growing number of evidence shows that some invertebrates possess an antiviral immunity parallel to the interferon (IFN) system of higher vertebrates. For example, the IRF (interferon regulatory factor)-Vago-JAK/STAT regulatory axis in an arthropod, shrimp Litopenaeus vannamei (whiteleg shrimp) is
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service