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S0266

Sigma-Aldrich

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

Synonym(s):

1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

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About This Item

Linear Formula:
NH2(CH2)3NH(CH2)4NH2
CAS Number:
124-20-9
Molecular Weight:
145.25
Beilstein:
1698591
EC Number:
204-689-0
MDL number:
MFCD00008229
UNSPSC Code:
12352200
PubChem Substance ID:
24899434
NACRES:
NA.52
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

product line

BioReagent

Assay

≥98%

form

solid or liquid

mol wt

145.25 (AMU)

technique(s)

cell culture | mammalian: suitable

refractive index

n20/D 1.479 (lit.)

density

0.925 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, and protease, none detected

storage temp.

room temp

SMILES string

NCCCCNCCCN

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

Gene Information

rat ... Ppm1a(24666)

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General description

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.[1]

Application

Spermidine has been used in the preparation of gold beads[2] and as a component in nuclei isolation buffer (NIB).[3]
Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.[4][5]

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Spermidine helps to maintain the proper activity of gene expression.[1] This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300).[6] Spermidine participates in cell growth.[7]

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

233.6 °F - closed cup

Flash Point(C)

112 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9651
BCCK8114
BCCK8113
BCCJ1130
BCCH8625

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Find documentation for the products that you have recently purchased in the Document Library.

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Organization, developmental dynamics, and evolution of plastid nucleoids
Sato N, et al.
International Review of Cytology, 232, 217-262 (2003)
Molecular Determinants of Metaplastic and Neoplastic Transformation in the Esophageal Epithelium
Progress in Molecular Biology and Translational Science, 96, 63-92 (2010)
Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs
Hogge GS, et al.
Cancer Gene Therapy, 6(1), 26-26 (1999)
Delivery of DNA to skin by particle bombardment.
Shixia Wang et al.
Methods in molecular biology (Clifton, N.J.), 245, 185-196 (2004-01-07)
Elumalai Sivamani et al.
Plant cell reports, 28(2), 213-221 (2008-11-19)
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded
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Questions

1–7 of 7 Questions  

  1. Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?

    1 answer

    1. The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
      1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
      2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045

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  2. What is the solvent that spermidine is dissolved in?

    1 answer

    1. This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.

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  3. How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?

    1 answer

    1. This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
      Molarity=Density x 1000 x Purity/MW

      This product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.

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  4. How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?

    1 answer

    1. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.

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  5. How frequently should new solutions be made?

    1 answer

    1. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.

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  6. Is this a spermidine solution or a neat liquid, and what is the concentration?

    1 answer

    1. Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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