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P8047

Sigma-Aldrich

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgG

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

phycoerythrin (R-PE) conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment R-Phycoerythrin antibody produced in goat has been used in bead-based assay and immunoassay multiplex magpix
Anti-Human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry . The antibody can also be used for direct immunofluorescence (1:32).

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises the H chain constant region.
IgG antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The use of anti-human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody helps avoid background staining due to the presence of Fc receptors. The product is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones λ myeloma proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optimization of a magnetic bead-based assay MAGPIX textregistered-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings
Varela ML, et al.
Malaria Journal, 17(1), 324-324 (2018)
Longitudinal analysis of antibody responses in symptomatic malaria cases do not mirror parasite transmission in peri-urban area of Cote d?Ivoire between 2010 and 2013
Koffi D, et al.
PLoS ONE, 12(2), e0172899-e0172899 (2017)
Dieter Palmberger et al.
PloS one, 7(4), e34226-e34226 (2012-04-10)
Recombinant production of therapeutically active proteins has become a central focus of contemporary life science research. These proteins are often produced in mammalian cells, in order to obtain products with post-translational modifications similar to their natural counterparts. However, in cases
William Spooner et al.
Nature communications, 9(1), 4128-4128 (2018-10-10)
Selecting the most appropriate protein sequences is critical for precision drug design. Here we describe Haplosaurus, a bioinformatic tool for computation of protein haplotypes. Haplosaurus computes protein haplotypes from pre-existing chromosomally-phased genomic variation data. Integration into the Ensembl resource provides
Kin-Mei Leung et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(11), 1722-1733 (2015-08-04)
FS102 is a HER2-specific Fcab (Fc fragment with antigen binding), which binds HER2 with high affinity and recognizes an epitope that does not overlap with those of trastuzumab or pertuzumab. In tumor cells that express high levels of HER2, FS102

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