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Merck

P1925

Protein A–Agarose macrobeads

aqueous suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
aqueous suspension
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form

aqueous suspension

Quality Level

extent of labeling

~6 mg per mL

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

~6 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

General description

Protein A is an immunoglobulin (Ig)-binding protein that might be suitable for low-pressure antibody isolation. It is used to purify large amounts of IgG and binds to the Fc part of the antibody at the CH2–CH3 interface. Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins.

Application

Protein A–Agarose macrobeads has been used in immunoprecipitation. It has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Physical form

Suspension in 0.9% NaCl containing 15Mm Sodium Azide


hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Human IgG Subclasses
Antibody Fc, 159-177 (2014)
B Blumbach et al.
Journal of cell science, 111 ( Pt 17), 2635-2644 (1998-08-14)
Porifera (sponges) are the oldest extant metazoan phylum. Dissociated sponge cells serve as a classic system to study processes of cell reaggregation. The reaggregation of dissociated cells is mediated by an extracellularly localized aggregation factor (AF), based on heterophilic interactions
C Cullin et al.
Yeast (Chichester, England), 10(1), 105-112 (1994-01-01)
In this paper are described a set of new high-copy-number yeast vectors, which are specially designed for the conditional expression of epitope-tagged proteins in vivo. One of the major advantages of these plasmids is that they allow polymerase chain reaction-amplified



Global Trade Item Number

SKUGTIN
P1925-5ML04061834357193
P1925-1ML04061834357186