Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

N3385

Sigma-Aldrich

N-Nitroso-N-ethylurea

ISOPAC®

Synonym(s):

ENU, N-Ethyl-N-nitrosourea

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C3H7N3O2
CAS Number:
759-73-9
Molecular Weight:
117.11
EC Number:
212-072-2
MDL number:
MFCD00053635
UNSPSC Code:
12352102
PubChem Substance ID:
24897640
NACRES:
NA.25
Pricing and availability is not currently available.

form

powder

storage temp.

−20°C

SMILES string

CCN(N=O)C(N)=O

InChI

1S/C3H7N3O2/c1-2-6(5-8)3(4)7/h2H2,1H3,(H2,4,7)

InChI key

FUSGACRLAFQQRL-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

N-Nitroso-N-ethylurea has been used as a mutation-inducing agent.[1][2][3]

Biochem/physiol Actions

DNA alkylating agent that is carcinogenic in many animal species. Induces benign and malignant tumors of numerous types, including the nervous tissue, stomach, esophagus, pancreas, respiratory tract, intestine, lymphoreticular tissues, skin, and kidney.

Packaging

Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.
1 vial contains 1 g of material calculated on anhydrous basis.

Caution

Injecting any compatible solvent permits preparation of any desired strength solution without exposure.

Reconstitution

Dissolving the contents in 100 mL of solvent yields a 1% solution.

Legal Information

Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany

ISOPAC of

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Carc. 1B - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCV0567
MKCT1606
MKCR6942
MKCN4387
MKCK3460

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Induction of mutations by ENU in the medaka germline.
Ishikawa Y
The fish biology journal Medaka, 10, 27-29 (1999)
Double cone dystrophy and RPE degeneration in the retina of the zebrafish gnn mutant.
Biehlmaier O, et al.
Investigative Ophthalmology & Visual Science, 44, 1287-1287 (2003)
Mouse mutagenesis using N-ethyl-N-nitrosourea (ENU).
Salinger AP and Justice MJ
Cold Spring Harbor Protocols, 4 (2008)
Jinjun Dang et al.
Blood, 125(23), 3609-3617 (2015-04-10)
Alterations of genes encoding transcriptional regulators of lymphoid development are a hallmark of B-progenitor acute lymphoblastic leukemia (B-ALL) and most commonly involve PAX5, encoding the DNA-binding transcription factor paired-box 5. The majority of PAX5 alterations in ALL are heterozygous, and
Georgina Caruana et al.
PloS one, 8(3), e55429-e55429 (2013-03-08)
Mice harbouring gene mutations that cause phenotypic abnormalities during organogenesis are invaluable tools for linking gene function to normal development and human disorders. To generate mouse models harbouring novel alleles that are involved in organogenesis we conducted a phenotype-driven, genome-wide
View All Related Papers

Articles

DNA Damage and Repair

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. I am writing to inquire about the conditions at which this product is shipped. Is it shipped on dry ice or with cool packs?

    1 answer

    1. The long term storage recommendation for this product is -20° C. However, the item is shipped at ambient temperature. Freezer storage products that ship at ambient temperature are stable for up to 2 weeks to allow for transit time. Items left at room temperature for short periods will not be adversely effected.

      Helpful?

  4. What is the pH of NEU solution made in DMSO

    1 answer

    1. The pH of this product in various solvents, such as DMSO, has not been determined. This information would have to be determined by the end user.

      Helpful?

  5. What solvent is generally used to dissolve ENU? DMSO is ok?

    1 answer

    1. The solubility of N3385 has been tested at 100 mg/mL in deuterated DMSO. The deuterated form of DMSO is more suitable for use in NMR, etc. The non-deuterated form of DMSO is sufficient for general use.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service