Adenosine 5′-triphosphate (ATP) assay mix

Name: Adenosine 5′-triphosphate (ATP) assay mix
Brand: SIGMA

lyophilized powder

Synonym(s):

ATP assay kit, ATP detection kit

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About This Item

NACRES:

NA.84

eCl@ss:

32160414

UNSPSC Code:

41116133

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Properties

form

lyophilized powder

Quality Segment

200

usage

1 vial sufficient for ≥50 assays

technique(s)

PCR: suitable, protein detection: suitable

storage temp.

−20°C

Description

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.

Preparation Note

Reconstitute with 5 ml of sterile water.

Other Notes

Contains luciferase, luciferin, MgSO₄, EDTA, DTT, BSA, and Tricine buffer.

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This Item	FLASC	FLAA	A3515
Sigma-Aldrich FLAAM Adenosine 5′-triphosphate (ATP) assay mix	Sigma-Aldrich FLASC Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit	Sigma-Aldrich FLAA Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit	Sigma-Aldrich A3515 Adenosine 2′,5′-diphosphate–Agarose
description lyophilized powder	description for cellular ATP determination	description for ATP quantitation	description lyophilized powder
usage vial sufficient for ≥50 assays	usage -	usage -	usage -
technique(s) PCR: suitable	technique(s) -	technique(s) -	technique(s) -
form lyophilized powder	form -	form -	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200