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Key Documents

EC11303

Sigma-Aldrich

Escherichia coli

Strain B, lyophilized cells

Synonym(s):

E. coli

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About This Item

MDL number:
MFCD00131026
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.

Quality Level

200

sterility

non-sterile (not processed or packaged aseptically)

form

lyophilized cells

suitability

not intended for starter culture

storage temp.

−20°C

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General description

Escherichia coli is a non-pathogenic, facultative anaerobic flora present in the intestine of human.[1] It belongs to the genus, Escherichia, which consists of motile gram-negative bacilli.[1]

Application

Escherichia coli has been used:
  • in the isolation of DNA from lyophilized cell[2]
  • in the preparation of crude cell lysate for developing scalable purification method for recombinant protein A[3]
  • as test proteome for comparative electrophoresis[4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000324914
0000305774
0000231256
0000220774
048M4031V

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Horizontal comparative fluorescence two-dimensional gel electrophoresis for improved spot coordinate detection
Hanneken M, et al.
Electrophoresis, 35(8), 1118-1121 (2014)
Severe atopic dermatitis is associated with a high burden of environmental Staphylococcus aureus
Leung AD, et al.
Clinical and Experimental Allergy, 38(5), 789-793 (2008)
Diarrheagenic Escherichia coli
Nataro JP and Kaper JB
Clinical Microbiology Reviews, 11(1), 142-201 (1998)
Accelerated recombinant protein purification process development: Automated, robotics-based integration of chromatographic purification and analysis
Londo T, et al.
Journal of Chromatography A, 798(1), 73-82 (1998)
A D Leung et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 38(5), 789-793 (2008-03-18)
About 90% of patients with atopic dermatitis (AD) are colonized with Staphylococcus aureus. S. aureus worsens AD by secreting superantigens and structural molecules within the cell wall that induce skin inflammation. Therefore, S. aureus in the home may contribute to
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Could you provide the reconstitution method and concentration for product EC11303 under the brand SIGMA?

    1 answer

    1. The cells in this product are expected to be viable and may grow in culture, but it's important to note that the product is not intended for culture use. The material is not handled aseptically during manufacturing, so it is likely that other organisms would be present. The main purpose of this product is to serve as a biomass, specifically for the extraction of biomolecules such as DNA and lipids. It's important to understand that there is no guarantee of viability or purity. If the end user wishes to reconstitute the product for culture, they should add 0.3 to 0.4 ml of suitable liquid medium/broth to the freeze-dried material and mix it well. Then, they should transfer the total mixture to a culture tube or plate containing 5 - 6 mL medium/broth. It's worth noting that there is no cell count estimate available for this product.

      Helpful?

  2. What is the method of reconstitution for this product and what is the concentration?

    1 answer

    1. These cells are expected to be viable and may grow in culture. However, the product is not intended for culture use. The material is not handled aseptically during manufacturing, therefore other organisms would likely be present. The product is intended to serve as a biomass, i.e., for the extraction of biomolecules such as DNA and lipids. There is no guarantee of viability or purity. Should the end user wish to reconstitute the product for culture, add to the freeze-dried material 0.3 to 0.4 ml of suitable liquid medium/broth and mix well. Transfer the total mixture to a culture tube or plate containing 5 - 6 mL medium/broth. There is no cell count estimate available.

      Helpful?

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