E2636

ExtrAvidin^® –Alkaline Phosphatase

buffered aqueous solution

• Synonym(s): Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00163600
• Form: buffered aqueous solution

Properties

• conjugate: alkaline phosphatase conjugate
• Quality Segment: 200
• form: buffered aqueous solution
• technique(s): dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[1] It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[2] ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin^® -Alkaline Phosphatase has been used in:

• Dot blot
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Indirect ELISA
• Enzyme linked immunosorbent assay (ELISA)[3][4]
• Enzyme-linked immunosorbent spot (ELISPOT) analysis[5]
• Competitive ELISA[6]

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable