DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Denhardt′s Solution has been used:
as a component in hybridization buffer for the hybridization of brain sections[1]
in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process[2]
Journal of biomolecular techniques : JBT, 14(3), 197-204 (2003-09-19)
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