Hep 3B Cell Line human

86062703, human liver, Epithelial

• Synonym(s): HEP3B217 Cells, Hep 3B2.1-7 Cells, Hep3B Cells
• UNSPSC Code: 41106514

Properties

• Product Name: Hep 3B Cell Line human, from human liver, 86062703
• biological source: human liver
• packaging: tube of 5 μg 86062703-DNA-5UG; pkg of vial of cells 86062703-1VL
• growth mode: Adherent
• karyotype: 2n = 46, modal no. 60
• morphology: Epithelial
• products: Alpha-foetoproteins, Hepatitis B Surface Antigen (HBSAg), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins
• receptors: Not specified
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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