Skip to Content
Merck
All Photos(1)

Key Documents

C3821

Sigma-Aldrich

Citrate-dextrose solution (ACD)

sterile-filtered

Synonym(s):

Acid citrate-dextrose, Anticoagulant citrate-dextrose

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
8013-89-6
MDL number:
MFCD00212913
UNSPSC Code:
12161700
NACRES:
NA.25
Pricing and availability is not currently available.

sterility

sterile-filtered

Quality Level

200

form

liquid

pH

4.5-5.5

storage temp.

2-8°C

Related Categories

General description

Citrate-dextrose solution (ACD) is an anticoagulant and preservative used routinely for blood storage. The ideal red blood cells shelf life is 21 days when stored in ACD solution.[1] Red blood cells in ACD solution utilize glucose and their viability is impairs when reinfused.[2]

Application

Citrate-dextrose solution (ACD) has been used for dilution of blood for haematological studies.[3] It has also been used as an anticoagulant during blood collection by cardiac puncture from mice[4] and human bone marrow isolation.[5]
For research use only. Not for use in diagnostic procedures.

Biochem/physiol Actions

Useful for anticoagulation of whole blood; erythrocyte survival

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000380975
0000334289
0000361083
0000367701
0000360022

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mingming Yang et al.
Experimental and therapeutic medicine, 20(2), 1253-1260 (2020-08-09)
The p53 gene is well known as a key tumor suppressor gene; it is vital for hematopoietic stem cell differentiation and growth. In the present study, the change of platelets (PLTs) in p53 knockout mice (p53-/- mice) was investigated. The
Isolation, expansion and characterisation of mesenchymal stem cells from human bone marrow, adipose tissue, umbilical cord blood and matrix: a comparative study
Secunda R, et al.
Cytotechnology, 67(5), 793-807 (2015)
Interaction between FOG-1 and the corepressor C-terminal binding protein is dispensable for normal erythropoiesis in vivo
Katz SG, et al.
Molecular and Cellular Biology, 22(9), 3121-3128 (2002)
Comparison of equine platelet function and survival in whole blood collected in acid-citrate-dextrose solution or citrate-phosphate-dextrose-adenine solution
Bozorgmanesh R, et al.
Veterinary Clinical Pathology / American Society For Veterinary Clinical Pathology, 46(2), 262-268 (2017)
Hideki Miyachi et al.
International journal of cardiology, 266, 61-66 (2018-06-12)
Acute thrombosis is a crucial cause of bioresorbable vascular graft (BVG) failure. Bone marrow-derived mononuclear cell (BM-MNC)-seeded BVGs demonstrated high graft patency, however, the effect of seeded BM-MNCs against thrombosis remains to be elucidated. Thus, we evaluated an antithrombotic effect
View All Related Papers

Questions

1–10 of 11 Questions  

  1. What is the composition of product C3821, Citrate-dextrose solution (ACD)?

    1 answer

    1. This corresponds to an ACD-A type solution. The composition of this product is as follows:
      1. 22.00 g/L C3434, Citric Acid, trisodium salt, dihydrate
      2. 7.30 g/L C0759, Citric Acid, anhydrous
      3.24.50 g/L G7528, D-(+) Glucose.

      Helpful?

  2. What is the composition of this citrate-dextrose solution? Is it solution A or B as defined by the US Pharmacopeia?

    1 answer

    1. The composition of the citrate-dextrose solution is as follows:
      1. 22.00 g/L Citric Acid, trisodium salt, dihydrate (C3434)
      2. 7.30 g/L Citric Acid, anhydrous (C0759)
      3. 24.50 g/L D-(+) Glucose (G7528)
      Based on this information, it is likely that the solution is ACD A, which is more commonly used than ACD B as defined by the US Pharmacopeia.

      Helpful?

  3. Is product C3821, Acid-Citrate-Dextrose, a concentrate? What ratio of product to whole blood is recommended?

    1 answer

    1. C3821 can be considered a stock solution, and the bottle can be used multiple times. The ratio of ACD or Citrate Dextrose solution can vary from one source to another. A ratio of 1:11 is the more concentrated usage recommended, although dilutions as high as 1:16 are sometimes found in the literature. A good compromise might be a 1:12 dilution.
      Given that there is no preservative in the solution, there is a possibility of contaminating the solution through routine use. Therefore, care should be taken to ensure that the solution does not become contaminated through routine use. The recommended storage temperature is 2-8°C.

      Helpful?

  4. Is the quality of the ACD solution affected if the bottles are left at ambient temperature (20°C in a temperature-controlled facility) for 48 hours, considering that they are typically required to be refrigerated?

    1 answer

    1. The recommended storage temperature for the ACD solution is between 2-8°C. There is no formal data to suggest that the product can be stored outside of this range without affecting its quality. Additionally, the product is capable of being shipped at ambient temperature for a duration of up to 14 days.

      Helpful?

  5. Can it used in prp preparation?

    1 answer

    1. This product is not tested for platelet-rich plasma preparation, however, Acid Citrate-Detrose is commonly used in this procedure. There is no reason to suspect that this item would not be appropriate. This product is intended for research use only and is not suitable for IVD or therapeutic use.

      Helpful?

  6. Hi, I want to order ACD-A solution, I found you have ACD (C3821), I wonder if you can provide the content and concentration of your ACD so that I can compare and see what´s difference between your ACD and ACD-A

    1 answer

    1. This product is equivalent to ACD- A solution.
      The composition is as follows:
      22.00 g/L C3434, Citric Acid, trisodium salt, dihydrate
      7.30 g/L C0759, Citric Acid, anhydrous
      24.50 g/L G7528, D-(+) Glucose (Dextrose)

      Helpful?

  7. HI, I have this product Dextrose solution C3821-10X50ML. How long could I let it at room temps. It was shipped and keeping in shiping for 3 days. Thanks, Chenier

    1 answer

    1. This product is shipped in ambient temperature. Once the product arrives to the address on the order, it is to be stored at 2-8°C by the end-user. Products shipped ambient but stored frozen or refrigerated should be stable in ambient temperature for up to two weeks. Please view this link for more information on Storage and Transport Conditions: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf.

      Helpful?

  8. At what concentration should Product C3821, Citrate-dextrose solution, be used?

    1 answer

    1. Use 1 part citrate dextrose solution to 9 parts blood.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. What is the composition of Product C3821, Citrate-dextrose solution?

    1 answer

    1. The composition if this product is 22.0 g/L C3434 (Citric Acid, trisodium salt, dihydrate); 7.3 g/L C0759 (Citric Acid, anhydrous); and 24.5 g/L G7528 (D-(+)-Glucose).

      Helpful?

1–10 of 11 Questions  

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service