The composition of the media can be found at the following link:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media#table
B5282
Bold Modified Basal Freshwater Nutrient Solution
50 ×, liquid, suitable for plant cell culture
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About This Item
NACRES:
NA.72
UNSPSC Code:
10171502
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Segment
form
liquid
concentration
50 ×
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Application
Bold Modified Basal Freshwater Nutrient Solution, 50x is a chemstock used to prepare Bold Modified Freshwater Nutrient Solution for use in phycology/algae studies.
Preparation Note
With major salts and trace elements. Modified with trace elements as requested for ASTM TOXICITY TESTS E1193 and E1295 conducted with Daphnia species.
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
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¿Podrían informarme de la composición de B5282, Bold Modified Basal Freshwater Nutrient Solution?
1 answer-
Helpful?
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Can this medium be combined with agar to make a solid medium?
1 answer-
While not tested as such, a gelling agent may be added to this solution to form a solid medium. As this is a prepared solution, the agar would be added directly to the medium.
Please see the link below, step 7, to review the general phycology media preparation protocol: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media
Helpful?
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Is this suitable for freshwater diatom culture?
1 answer-
This product is tested for culture using green algae that are not specifically designated as diatomaceous. This application may need to be validated by the end user. The formulation is prepared according to the the reference below:
Nichols, H. W. Growth Media-Freshwater in: Stein, J.r. (ed) Phycological Methods, Cambridge Univ. Press, 7-23, 1973.Helpful?
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