Unforunately we do not have information on the cross-reactivity of the antibody B3648-.5ml with the cynomolgus. Additionally, no in-house epitope mapping has been conducted on this antibody. However, Robert G. Hamilton and Sherie L. Morrison, in their 1993 article in the Journal of Immunological Methods, have performed some further characterization. According to the article, "The anti-human IgG4 MAbs (HP6023, HP6025) bound to a similar epitope on the carboxyl terminus of CH2 or the CH3 of human IgG4." For more details, please refer to the article: "Journal of Immunological Methods Volume 158, Issue 1, 14, Pages 107-122."
B3648
Anti-Human IgG4−Biotin antibody, Mouse monoclonal
clone HP-6025, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Human IgG4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
HP-6025, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:60,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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Specificity
Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.
Application
Anti-Human IgG4−Biotin antibody, Mouse monoclonal has been used in immunohistochemistry and immunostaining.
Monoclonal Anti-Human IgG4-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:15,000. It was used for detection of serum IgG4 produced in response to malaria parasite infection by ELISA.
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG4 antibody allows for the binding of various labels such as avidin or streptavidin.
IgG4 is the least abundant immunoglobulin present in the human serum. IgG4 is implicated in a unique process called fab-arm exchange (FAE) and limits the formation of immune complex. It has anti-inflammatory properties. IgG4 functions as a therapeutic antibody. It provides protection against allergy, prevents mast cell degranulation and has a harmful role in malignant melanoma. IgG4 may be associated with autoimmune diseases such as, autoimmune pancreatitis (AIP) type 1 and 2, Mikulicz syndrome, Kuttner′s tumor, Riedel′s thyroiditis and tubulointerstitial nephritis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Does the antibody B3648-.5ml cross-react with the cynomolgus monkey?
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