A2289
Albumin, Tetramethylrhodamine isothiocyanate bovine
lyophilized powder
Synonym(s):
TRITC-albumin
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About This Item
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biological source
bovine
Quality Level
Assay
≥90% (Lowry)
form
lyophilized powder
extent of labeling
≥0.5 mol TRITC per mol albumin
technique(s)
cell culture | mammalian: suitable
impurities
salt, free
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).
Application
Albumin, Tetramethylrhodamine isothiocyanate has been used:
- in photoinduced release assay of polyethylene beads
- for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
- in fluorescent correlation spectroscopy
Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
Preparation Note
Prepared from crystallized and lyophilized albumin (A4378).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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