A0410
Aminoallyl-dUTP sodium salt
≥85%, lyophilized powder
Synonym(s):
5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
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About This Item
Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
Molecular Weight:
523.22 (free acid basis)
UNSPSC Code:
41106305
NACRES:
NA.51
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Application
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
- to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
- for the generation of chromatin immunoprecipitation (ChIP) probes
- in the reverse transcription of RNA for the synthesis of fluorescent labelled probes
Biochem/physiol Actions
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
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Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Cox W G and Singer V L
Biotechniques, 36(1), 114-122 (2004)
Miguel A Matilla et al.
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Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). A global analysis of Pseudomonas putida genes expressed during their interaction
Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.
Dirsch O, Ji Y, Bohr J, et al.
Appl. Immunohistochem., 15, 332-337 (2007)
W Gregory Cox et al.
BioTechniques, 36(1), 114-122 (2004-01-27)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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