This product has not been tested for use on Corning Transwell plates. The principle of that assay should make it conducive to use with any contained cell bodies, so it would be expected to work. For further guidance, please refer to academic literature. The link provide below may be of interest:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C26&q=%2296992%22+%22transwell%22&btnG=
96992
Cell Counting Kit - 8
for quantitation of viable cell number in proliferation and cytotoxicity assays
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352108
Pricing and availability is not currently available.
Quality Segment
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
protein detection: suitable
color
lightdeep red
pH
6-8
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
Application
Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
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This Item | |||
|---|---|---|---|
| description for quantitation of viable cell number in proliferation and cytotoxicity assays | description ≥97% (HPLC) | description suitable for cell analysis | description ≥90% (HPLC) |
| shipped in ambient | shipped in - | shipped in ambient | shipped in - |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C (−15°C to −25°C) |
| color lightdeep red | color - | color - | color - |
| solubility water: soluble | solubility - | solubility - | solubility - |
| pH 6-8 | pH - | pH - | pH - |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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This kit is it usefull for cell culture on transwell ?
1 answer-
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How, much volume to be used for 12 well plate. For e.g I am growing cells in my 12 well plate over a thin film. some paper mentions to use 10%, so if I add 100ul with 1ml media. will it work?
1 answer-
Yes, 10% of the culture volume is suitable for use. The kit instructions require 10uL of reagent per 100ul culture volume in a 96 well format. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdf
Please see the following link to review troubleshooting tips that may also be of interest:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdfHelpful?
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What is the solution volume provided in the "3000 tests"?
1 answer-
The 96992-3000TESTS vial will include 30 milliliters of reagent.
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100 Test means 100 wells, so only one 96-well plate?
1 answer-
The reagents provided in kit 96992 are sufficient for 100 tests. A 96 well microplate is not included with the kit. The customer must provide the 96 well plate. Although a solid 96 well plate can be used, a 96 well microplate with removable strips will allow for the most efficient use of the kit.
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How much volume (in milliliters) is present in each vial of the CCK-8 solution?
1 answer-
The volume supplied for Product No. 96992, Cell Counting Kit, is as follows:
•100 tests: 1 ml x 1 vial
•500 tests: 5 ml x 1 vial
•3000 tests: 5 ml x 6 vialsHelpful?
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What are the volumes of reagents included in the 96992-100test-F kit?
1 answer-
Supplier information specifies that the kit contains 6 bottles, each containing 5 ml of solution. Based on the product datasheet, each well of a well-plate requires 10 microliters of solution, indicating that for 3000 tests, the kit should contain 30 ml of solution. Using these calculations, for the 100 test kit, each bottle would contain approximately 167 microliters of solution (5 ml divided by 30 = 167 microliters).
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Is this kit suitable for use with HEK293 or HEK293f cells?
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Yes, the 96992 kit can be utilized to stain both HEK293 and HEK293B cells. The Cell Count kit is not cell type-specific and is expected to function effectively for counting viable mammalian cells. In this kit, the WST-8 dye, which is a formazan stain is specific to viable cells and is not present in nonviable cells.
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Hi, what is the solution volume provided in the "500 tests" SKU? Thanks in advance, Aviv.
1 answer-
The 500 test volume is 5 mL. See the links below to the Product datasheet and the Product Troubleshooting Guide for additional information.
Product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdfTroubleshooting Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdfHelpful?
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