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59747

Sigma-Aldrich

Lactic Dehydrogenase, recombinant from E. coli

≥90 U/mg

Synonym(s):

(S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate

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About This Item

CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in E. coli

Quality Level

100

form

powder

specific activity

≥90 U/mg

storage temp.

−20°C

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General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms.[1] LDH possess a tetrameric structure.[2]

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:
  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays[3]
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay[4][5]
  • in in vitro DltC D-alanylation assay[6]

Biochem/physiol Actions

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.[2]

Unit Definition

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL9892
BCCK5085
BCCH0280
BCCG1380
BCCC3043

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Cardiac, Vascular, and Skeletal Muscle Systems
Haschek and Rousseaux's Handbook of Toxicologic Pathology (2013)
Circulating biomarkers in malignant melanoma
Advances in Clinical Chemistry, 69, 47-89 (2015)
An atypical glycolysis in Clostridium thermocellum
Zhou J, et al.
Applied and Environmental Microbiology, 39, AEM-04037 (2013)
A partial reconstitution implicates DltD in catalyzing lipoteichoic acid D-alanylation
Wood BM, et al.
The Journal of Biological Chemistry, 293(46), 17985-17996 (2018)
Glycolysis without pyruvate kinase in Clostridium thermocellum
Olson DG, et al.
Metabolic engineering, 39, 169-180 (2017)
View All Related Papers

Questions

1–3 of 3 Questions  

  1. Can you provide more information on the biological source for recombinant LDH 59747? Does the certificate of origin specify whether the recombinant protein is from mice or another source? It seems that the certificate of origin does not offer detailed information to clarify this.

    1 answer

    1. The material is Lactate dehydrogenase from chicken heart, expressed in recombinant microbes.

      Helpful?

  2. I want to know the molecular weight of this protein

    1 answer

    1. This product is a recombinant enzyme from E. coli. The species and tissue of origin is the chicken heart. The molecular weight of this material has not been determined. However, according to Huston et al. 1972, Lactate Dehydrogenase from the bovine heart has a molecular weight of approximately 136,700 daltons.

      Helpful?

  3. What species is Product No. 59747, Lactic Dehydrogenase, recombinant from E. coli?

    1 answer

    1. This product is a recombinant Lactic Dehydrogenase from chicken heart made in E. coli.

      Helpful?

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