08124

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥10,000,000 units/mg protein

• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.2.1.68 (BRENDA, IUBMB)
• MDL number: MFCD00131418
• Specific activity: ≥10,000,000 units/mg protein

Properties

• form: ammonium sulfate suspension
• Quality Level: 100
• specific activity: ≥10,000,000 units/mg protein
• storage temp.: 2-8°C

Description

Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Biochem/physiol Actions

Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.

Analysis Note

≥10 MU/mg protein

Other Notes

One unit causes an increase in A₆₁₀ of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H411
• pcodes: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable