Sudan Black B is not a fluorescent dye. Sudan Black B has been used to block autofluorescence. The following information is available from the website. Although the reference below is specific to brain tissue, the expectation is that Sudan Black B should be able to be used for a variety of different tissue types. There is no specific information available for paraffin sections of eyeballs.
Sudan Black B treatment reduces autofluorescence and improves the resolution of in situ hybridization-specific fluorescent signals of brain sections.
Histology and histopathology (2010-06-17)
V C Oliveira, R C V Carrara, D L C Simoes, F P Saggioro, C G Carlotti, D T Covas, L Neder
PMID20552552
ABSTRACT
Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels.
