SCT024
BioTracker 543 Yellow β-Gal Dye
Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).
Synonym(s):
Live cell imaging probe
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
eCl@ss:
32161000
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).
The BioTracker 543 Yellow β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.
Spectral Properties
Absorbance: 525nm (488nm compatible)
Emission: 543nm
The BioTracker 543 Yellow β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.
Spectral Properties
Absorbance: 525nm (488nm compatible)
Emission: 543nm
Preparation Note
Store BioTracker 543 Yellow β-Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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