This has not been determined. As with most fluorescent dyes, it will fade over time. See the link below. This reference may offer additional helpful information.
https://www.biorxiv.org/content/10.1101/2021.12.15.472742v1.full.pdf
SCT021
BioTracker 609 Red Ca2+ AM Dye
Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications.
Synonym(s):
Live cell calcium detection probe, Live cell imaging probe, Red fluorescent calcium indicator
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.47
Pricing and availability is not currently available.
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technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
General description
Calcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.
The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.
Spectral Properties
Absorbance: 595nm
Emission: 609nm
The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.
Spectral Properties
Absorbance: 595nm
Emission: 609nm
Storage and Stability
Store BioTracker 609 Red Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
1202.4 °F
Flash Point(C)
650.2 °C
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