MABC1132
Anti-PD-1 Antibody, clone G4
clone G4, from hamster(Armenian)
Synonym(s):
Programmed cell death protein 1, Protein PD-1, mPD-1, CD279
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source
hamster (Armenian)
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
G4, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
flow cytometry: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Pdcd1(18566)
Related Categories
General description
Protein PD-1, mPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 18566) in murine species. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 21-169), a transmembrane domain (aa 170-190), and a cytoplasmic domain (aa 191-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain (aa 31-139). PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-1 lacks SH2- or SH3-binding motifs on its cytoplasmic tail, but contains the N-terminal sequence VDYGEL that forms an immunoreceptor tyrosine-based inhibition motif (V/I/LxYxxL), which recruits SH2 domain-containing phosphatases. The cytoplasmic tail also contains the C-terminal sequence TEYATI, which forms an immunoreceptor tyrosine-based switch motif (TxYxxL). PD-1 ligation is reported to inhibit the activation of T-cell receptor proximal kinases, which results in attenuation of Lck-mediated phosphorylation of ZAP-70 and initiation of downstream events. PD-1 is also reported to impair the activation of the MEK-ERK MAP kinase pathway by inhibiting activation of PLC- 1 and Ras.
Specificity
Clone G4 is an Armenian Hamster monoclonal antibody that specifically detects PD-1 in murine cells.
Immunogen
Murine PD-1Ig fusion protein.
Application
Affects Function Analysis: A representative lot detected PD-1 in Affects Funtion applications (Dronca, R.S., et. al. (2016). JCI Insight. 1(6); Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Overacre-Delgoffe, A.E., et. al. (2017). Cell. 169(6):1130; Tsushima, F., et. al. (2006). Blood. 110(1):180-5).
Flow Cytometry Analysis: A representative lot detected PD-1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96).
Flow Cytometry Analysis: A representative lot detected PD-1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96).
Detects Programmed cell death protein 1 using this armenian hamster monoclonal Anti-PD-1, clone G4, Cat. No. MABC1132, testted for use in Flow Cytometry and Affects Function.
Quality
Evaluated by Flow Cytometry in EL4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected PD-1 in 1X10E6 EL4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected PD-1 in 1X10E6 EL4 T lymphoma cells.
Target description
~31.84 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Ti Wen et al.
Cancer immunology research, 10(2), 162-181 (2021-12-17)
Cytotoxic CD8+ T cells (CTL) are a crucial component of the immune system notable for their ability to eliminate rapidly proliferating malignant cells. However, the T-cell intrinsic factors required for human CTLs to accomplish highly efficient antitumor cytotoxicity are not
Chunhua Chen et al.
JCI insight, 6(8) (2021-04-23)
Although the immune checkpoint role of programmed death ligand 1 (PD-L1) has been established and targeted in cancer immunotherapy, the tumor-intrinsic role of PD-L1 is less appreciated in tumor biology and therapeutics development, partly because of the incomplete mechanistic understanding.
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