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Sigma-Aldrich

Donkey Anti-Goat IgG Antibody, Rhodamine conjugate, Species Adsorbed

Chemicon®, from donkey

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

TRITC conjugate
rhodamine conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

goat

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Goat IgG

WAVELENGTH:

Absorption peak = 550 nm, Emission peak = 570 nm.

Application

Donkey anti-Goat IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:50-1:200 Optimal working dilutions must be determined by end user.

Physical form

Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.1% sodium azide.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

RHODAMINE/PROTEIN:

Approximately 0.5 (A550/A280).

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hirohumi Suzuki et al.
Acta histochemica et cytochemica, 48(6), 173-178 (2016-02-09)
Immunohistochemical techniques were employed to investigate the distribution of the chemokine CXCL14, in the mouse pancreas. CXCL14-immunoreactive cells were detected in the peripheral region of the pancreatic islets and were immunoreactive for somatostatin, but not for glucagon, insulin, and pancreatic
H Suzuki et al.
Anatomia, histologia, embryologia, 47(2), 159-166 (2018-01-10)
Immunohistochemical techniques were employed to investigate the distribution of amylin-like immunoreactive cells in the pancreas of gecko Homopholis fasciata. Four types of endocrine cells were distinguished: insulin immunoreactive (B cells), pancreatic polypeptide immunoreactive (PP cells), glucagon and pancreatic polypeptide immunoreactive
Fuminori Tanihara et al.
Animal science journal = Nihon chikusan Gakkaiho, 85(4), 395-404 (2014-01-24)
The zona pellucida (ZP) is considered to play important roles in the prevention of polyspermy in mammalian oocytes. However, in pigs we have shown that the presence of the ZP accelerates sperm penetration into the ooplasm during in vitro fertilization
Hirohumi Suzuki et al.
The Journal of veterinary medical science, 82(4), 408-413 (2020-02-11)
The distribution pattern of chemokine CXCL14-immunoreactive cells was examined by immunohistochemistry in the pituitary of the gecko Hemidactylus platyurus. Immunoreactive cells were observed in the pars intermedia and pars distalis of the pituitary, but not in the pars nervosa. All
Gian Marco Tosi et al.
Investigative ophthalmology & visual science, 61(8), 34-34 (2020-07-28)
This work was aimed to further characterize cells of idiopathic epiretinal membranes (iERMs). We wanted to determine the contribution of 90-kDa heat shock protein (HSP90) to sustain the transforming growth factor-β (TGF-β)-mediated signal transduction pathway in iERM. Immunofluorescence and confocal

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