ABE419
Anti-Histone H3.3 Antibody, K27M mutant
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H3.1, Histone H3.3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
mutation (Lys27Met)
Gene Information
human ... H3F3B(3021)
General description
Histone H3.3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3.3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
Specificity
This antibody recognizes Histone H3.3 with K27M mutation.
Immunogen
Epitope: Histsone H3 sequence surrounding K27M mutation
KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3.3 with K27M mutation.
Application
Anti-Histone H3.3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: A representative lot detected histone H3.30 K27M mutant in diffuse intrinsic pontice Glioma tissue lysates expressing Histone H3.3 K27M mutant. (Lewis, P. W., et al. (2013). Science. 340(6134):857-861.
Chromatin Immunoprecipitation: A representative lot co-precipitated chromatin fragments containing the promoter regions of ACTA and CCT8 genes from HEK293T transfectants expressing FLAG-HA-tagged histone H3.3 with K27M mutation (Peter W. Lewis and David Allis, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY).
Chromatin Immunoprecipitation: A representative lot co-precipitated chromatin fragments containing the promoter regions of ACTA and CCT8 genes from HEK293T transfectants expressing FLAG-HA-tagged histone H3.3 with K27M mutation (Peter W. Lewis and David Allis, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY).
Quality
Evaluated by Western Blotting in MEF (H3.3) and MEF (H3.3 K27M-HA-Flag) transfected cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected FLAG-HA-tagged K27M mutant, but not wildtype, histone H3.3 in 3x10E5 cell equivalent of lysate from MEF transfectants.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected FLAG-HA-tagged K27M mutant, but not wildtype, histone H3.3 in 3x10E5 cell equivalent of lysate from MEF transfectants.
Target description
~17 kDa observed
Physical form
Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Mélanie Pagès et al.
Brain pathology (Zurich, Switzerland), 28(1), 103-111 (2016-12-17)
Ganglioglioma (GG) is a grade I tumor characterized by alterations in the MAPK pathway, including BRAF V600E mutation. Recently, diffuse midline glioma with an H3 K27M mutation was added to the WHO 2016 classification as a new grade IV entity.
Hamid Nikbakht et al.
Nature communications, 7, 11185-11185 (2016-04-07)
Diffuse Intrinsic Pontine Gliomas (DIPGs) are deadly paediatric brain tumours where needle biopsies help guide diagnosis and targeted therapies. To address spatial heterogeneity, here we analyse 134 specimens from various neuroanatomical structures of whole autopsy brains from nine DIPG patients.
Thomas J Stone et al.
Acta neuropathologica, 135(1), 115-129 (2017-10-24)
Glioneuronal tumours are an important cause of treatment-resistant epilepsy. Subtypes of tumour are often poorly discriminated by histological features and may be difficult to diagnose due to a lack of robust diagnostic tools. This is illustrated by marked variability in
Fausto J Rodriguez et al.
Brain pathology (Zurich, Switzerland), 29(1), 126-140 (2018-09-08)
Anaplasia may be identified in a subset of tumors with a presumed pilocytic astrocytoma (PA) component or piloid features, which may be associated with aggressive behavior, but the biologic basis of this change remains unclear. Fifty-seven resections from 36 patients
Specific detection of methionine 27 mutation in histone 3 variants (H3K27M) in fixed tissue from high-grade astrocytomas.
Bechet, D; Gielen, GG; Korshunov, A; Pfister, SM; Rousso, C; Faury, D; Fiset et al.
Acta neuropathologica null
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