524604

Phosphatase, Alkaline, Human Placenta

• Synonym(s): Phosphatase, Alkaline, Human Placenta
• CAS Number: 9001-78-9
• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• form: liquid
• Quality Level: 100
• specific activity: ≥100 U/mg; ≥300 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native alkaline phosphatase from human placenta.

Native alkaline phosphatase from human placenta. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in the hydrolysis of 5′-terminal phosphates of DNA and RNA. Active site contains a reactive serine.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmol pNPP per min at 37°C, pH 10.15.

Physical form

In 10 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 50% glycerol, pH 7.6.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Other Notes

Fishman, W.H., et al. 1973. Anal. Biochem.51, 368.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1