264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

• Synonym(s): Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
• Empirical Formula (Hill Notation): C₃₆H₃₈F₃N₅O₁₄
• CAS Number: 1177935-21-5
• Molecular Weight: 821.71
• MDL number: MFCD01074977
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white to off-white
• solubility: DMSO: 10 mg/mL
• fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)OCc3ccccc3)CC(=O)OC)CCC(=O)OC)C(C)C)CC(=O)OC
• InChI key: NLDGPABVDARZCY-KIAQLKRMSA-N

Description

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Asp-Glu-Val-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable