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1.50550

Supelco

LiChrospher® RP-18 endcapped (5 µm) HPLC Columns

L x I.D. 250 mm x 4.6 mm Hibar® RT HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Pricing and availability is not currently available.

Product Name

LiChrospher® RP-18 endcapped (5µm) Hibar® RT 250-4.6, suitable for HPLC

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

LiChrospher®

feature

endcapped

packaging

1 ea of

extent of labeling

21.6% Carbon loading

parameter

400 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

25 cm × 4.6 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

General description

HPLC column
LiChrospher® RP-18 endcapped is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 endcapped reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 250-4.6 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Analysis Note

Pressure: ≤ 100 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

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Questions

  1. Are the following columns available for a US method: 4 mm x 30 cm and 4.0 mm x 15 cm, both packed with L1?

    1 answer
    1. The 4 mm x 30 cm USP L1 HPLC column is not offered due to the high back pressure created by 30 cm columns, which can overpressure systems with a typical pressure limit of 4000 PSI, even with a 5um packing. However, a 15 cm x 4mm ID C18 (USP L1) column is available, and two columns can be coupled together to make a 30 cm column. There are two types of HPLC Column Couplers with 10.32 threaded fittings. Coupler 54986 is a standard PEEK 10.32 to 10.32 Coupler that can be used with any column with 10.32 end fittings. HPLC Column Coupler 55213 is used to couple two columns together, which butts the two columns head-to-head.

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