Glycosylation and phosphorylation are important post-translational modifications in biological processes and biomarker research. The difficulty in analyzing these modifications is mainly their low abundance and dissociation of labile regions such as sialic acids or phosphate groups. One solution in matrix-assisted
Mass spectrometry reviews, 37(5), 652-680 (2017-12-12)
Sialic acids are a family of structurally unique and negatively charged nine-carbon sugars, normally found at the terminal positions of glycan chains on glycoproteins and glycolipids. The glycosylation of proteins is a universal post-translational modification in eukaryotic species and regulates
This paper reports an improved method for the identification of Acacia gum in cultural heritage samples using matrix assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) after enzymatic digestion of the polysaccharide component. The analytical strategy was optimized using a
Journal of the American Society for Mass Spectrometry, 31(6), 1180-1188 (2020-04-17)
Polyrotaxane (PR) is a necklace-like supramolecule composed of cyclic components, such as cyclodextrin (CD), and a threading polymer capped with bulky end groups. PR exhibits peculiar mechanical properties attributed to the intermolecular cross-links with CD. Various CD molecules threaded on
Journal of proteome research, 19(10), 3890-3905 (2020-09-08)
Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of N-linked and O-linked glycans in disease states. Robust analytical methods must be defined to identify suitable glycan biomarkers and glyco-therapeutics. Fortunately, in N-glycan analysis, a universal
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