Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

219215

Sigma-Aldrich

Leucocrystal Violet

Synonym(s):

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Linear Formula:
CH[C6H4N(CH3)2]3
CAS Number:
603-48-5
Molecular Weight:
373.53
EC Number:
210-043-9
MDL number:
MFCD00008314
UNSPSC Code:
12171500
PubChem Substance ID:
24853114
NACRES:
NA.47
Pricing and availability is not currently available.

form

powder

Quality Level

200

technique(s)

microbe id | staining: suitable

mp

175-177 °C (lit.)

λmax

260 nm

ε (extinction coefficient)

≥700 at 257-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

InChI key

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.[1]

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.[2][3]

Caution

Photo-ionization can cause color variations

related product

Product No.
Description
Pricing

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000236216
0000236216
MKBW1177V
MKBR8820V
MKBR8440V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

D R Doerge et al.
Rapid communications in mass spectrometry : RCM, 10(12), 1479-1484 (1996-01-01)
Gentian violet (GV) is a triphenylmethane dye antiseptic with potential for illegal use in livestock production, especially aquaculture where the related malachite green has been widely used. This potential misuse has regulatory importance because of the observed rodent carcinogenicity of
Detection Method for Histamine-Producing, Dairy-Related
Bacteria using Diamine Oxidase and Leucocrystal Violet
SUMNER S S
Journal of Food Protection, 52, 105-108 (1989)
Use of leuco crystal violet to enhance shoe prints in blood.
Bodziak WJ
Forensic Science International, 82, 45-52 (1996)
L G Rushing et al.
Journal of chromatography. B, Biomedical sciences and applications, 688(2), 325-330 (1997-01-24)
A sensitive analytical procedure for the determination of residues of leucomalachite green (LMG)-malachite green (MG) and leucogentian violet (LGV)-gentian violet (GV) in catfish or trout tissue is presented. Frozen (-20 degrees C) fish fillets were cut into small pieces and
Craig A. Coppock
Contrast: An Investigator's Basic Reference Guide to Fingerprint Identification Concepts (2007)
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Is 219215-5G the same powder that is used in the urinalysis dip sticks?

    1 answer

    1. Leucocrystal violet is the completely reduced form of crystal violet. The dye powder appears white to gray to lavender, and solutions are expected to be colorless. In addition to identifying lactobacilli in cheese, Leucocrystal Violet (LCV) is a cationic triarylmethane dye that has an affinity for both cellulosic and proteinaceous materials. It reacts with the hemoglobin in blood and turns the blood impression to a purple/violet color. Consequently, the dye is widely used to stain blood residue on both porous and non-porous materials. There is also mention of Leucocrystal Violet being used as an indicator on a urinalysis test strip, potentially to detect the presence of blood or protein. However, the quality level for Leucocrystal Violet is 200 on a scale of 100 to 600, and it is only advertised as suitable for Research Use Only. Urinalysis test strips sold for human use are classified as medical devices and are stated to be suitable for In Vitro Diagnostic Use. As such, product 219215 is not suitability tested to ensure the dye is suitble for use in the manufacture of urinalysis test strips.

      Helpful?

  2. What is the purity of lot/batch #:MKBW1177V

    1 answer

    1. The purity is not determined for this product, only carbon and nitrogen are quantitated.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service