53577-U

Ascentis^® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 7.5 cm × 3 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core PFP HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 7.5 cm × 3 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 7.5 cm × 3 mm
• surface area: 135 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 2.7 μm
• pore size: 90 Å pore size
• operating pH: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study utilizes the Ascentis^® Express F5, 2.7 µm HPLC Column to develop a robust method for the determination of lumacaftor in the presence of ivacaftor, identifying five novel degradation products. The high resolution and efficiency of the column were crucial for the detailed analysis and identification process. (Özcan et al., 2023).


• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: The Ascentis^® Express F5 column was employed to develop stability-indicating methods for tasimelteon, ensuring accurate determination and high-resolution identification of degradation products, highlighting the column′s effectiveness in stability studies and complex sample matrices. (Özcan et al., 2020).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns like the Ascentis^® Express F5. The article elaborates on the performance improvements and applications in analytical chemistry, emphasizing the column′s role in enhancing speed and resolution. (Ali et al., 2012).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable