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SRP3142

Sigma-Aldrich

PF-4 (CXCL4) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

CXCL4, Ironplact, Oncostatin A, Platelet Factor-4

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-10.0 ng/mL

mol wt

7.8 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

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S093000011624871235820
grade

pharmaceutical primary standard

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pharmaceutical primary standard

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pharmaceutical primary standard

grade

pharmaceutical primary standard

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

format

neat

format

neat

format

neat

format

neat

API family

divalproex

API family

sodium valproate

API family

dalteparin

API family

enoxaparin

General description

Platelet factor-4 (PF-4) is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets.[1] The gene encoding this protein is localized on human chromosome 4.[2] Recombinant human PF-4 is a 7.8kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

Biochem/physiol Actions

Platelet factor-4 (PF-4) is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis.[3] This protein associates with heparin and neutralizes it. It also neutralizes various negatively charged proteoglycans.[4]

Sequence

EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    Chemokines and Chemokine Receptors
    Manel Juan
    Wiley Guide to Chemical Incompatibilities (2013)
    B Scheuerer et al.
    Blood, 95(4), 1158-1166 (2000-02-09)
    Unstimulated monocytes rapidly undergo physiological changes resulting in programmed cell death (apoptosis) while stimuli promoting differentiation of these cells into macrophages were shown to inhibit apoptotic processes. In the present study, we report that the platelet-derived alpha-chemokine platelet factor 4
    S S Huang et al.
    The Journal of biological chemistry, 257(19), 11546-11550 (1982-10-10)
    A large scale purification procedure for the human platelet factor 4 proteoglycan carrier molecule has been developed. A yield of 46% and a 33,000-fold purification have been achieved, using poly-L-lysine-Sepharose affinity column chromatography, PF4-agarose affinity column chromatography, and Bio-Gel A-0.5m
    CXCL4-platelet factor 4, heparin-induced thrombocytopenia and cancer.
    Sandset PM
    Thrombosis Research, 129, S97-100 (2012)
    Yong Du et al.
    The Journal of experimental medicine, 219(7) (2022-06-01)
    Chemokines control the migratory patterns and positioning of immune cells to organize immune responses to pathogens. However, many chemokines have been associated with systemic autoimmune diseases that have chronic IFN signatures. We report that a series of chemokines, including CXCL4

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