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P2949

Sigma-Aldrich

PIPES sodium salt

≥99% (titration)

Synonym(s):

Na-PIPES, Piperazine-1,4-diethanesulfonate sodium salt, 1,4-Piperazinediethanesulfonic acid sodium salt, Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt

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About This Item

Linear Formula:
C8H17N2O6S2Na
CAS Number:
Molecular Weight:
324.35
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99% (titration)

form

crystalline powder

pH

4.5-4.7

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

0.1 M NaOH: 250 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

[Na+].OS(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1

InChI key

OGGAIRCLBMGXCZ-UHFFFAOYSA-M

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General description

1, 4-piperazinediethanesulfonic acid (PIPES) is a zwitterionic buffer that belongs to the group of the ethanesulfonic acid buffer series, first introduced by Good et al., It is a non-toxic buffer used in cell culture and tissue fixation for electron microscopy and other biological applications. PIPES don’t interact with metal ions. Since its pKa at 37°C is near physiological pH, it has been used as a buffering substance in biological, biochemical, and environmental studies. PIPES is developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Buffers can be prepared by adding a solution of the base to PIPES-free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Application

PIPES sodium salt buffer has been used:

  • to prepare BRB80 buffer for imaging dynamic microtubules and single kinesin molecules through fluorescence microscopy
  • as a component of PEM fixation solution
  • to reconstitute red blood cells (RBCs) to study the effect of pH on 5-coordinate alpha-nitrosyl-hemoglobin (HbNO) stability

Features and Benefits

  • Non-toxic
  • Easy to use
  • Thermally stable
  • Easy to handle and store

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Conventional specimen preparation techniques for transmission electron microscopy of cultured cells
Bozzola JJ
Methods in Molecular Biology, 1-18 (2007)
Catalytic application of 1, 4-piperazinediethanesulfonic acid (PIPES) for the one-pot multicomponent synthesis of pyrano [4, 3-b] pyrans
Organic preparations and procedures international, 52(4), 368-373 (2020)
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
Tuna Y, et al.
Journal of Visualized Experiments (2022)
Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation
Nie J, et al.
BMC Developmental Biology (2015)
Keity J Farfán-Pira et al.
microPublication biology, 2022 (2022-07-06)
In Drosophila , the pattern of the wing selector gene, vestigial ( vg ), is established by at least two enhancers: the Boundary Enhancer, which drives expression along the disc's Dorsal-Ventral boundary; and the Quadrant Enhancer (QE) that patterns the

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