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F1672

Sigma-Aldrich

Anti-Fibroblast Growth Factor-9 antibody, Mouse monoclonal

clone FG9-77, purified from hybridoma cell culture

Synonym(s):

Anti-FGF-9, Anti-GAF, Anti-HBFG-9, Anti-HBGF-9, Anti-SYNS3

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FG9-77, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

mouse, human

technique(s)

dot blot: 5 μg/mL using recombinant human FGF-9
indirect ELISA: suitable
microarray: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGF9(2254)
mouse ... Fgf9(14180)

General description

Fibroblast Growth Factor-9 (FGF-9) also refers as glia activating factor is a glycosylated single polypeptide of 208 amino acids that belongs to heparin-binding growth factors family. It has a crucial role in the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, rat pheochromacytoma cell line PC-12 and Balb/c 3T3 fibroblasts. It mainly expresses in brain and kidney. Monoclonal Anti-Fibroblast Growth Factor-9 antibody can be used for studying the fibroblast growth factors and their properties as well as to compare its expression pattern with physiological functions or pathological conditions. It can also be used in immunoblotting.

Specificity

The antibody reacts specifically with recombinant FGF-9. It does not cross-react with human acidic FGF or basic FGF. Mouse anti-Fibroblast Growth Factor-9 antibody reacts specifically with recombinant FGF-9.

Immunogen

recombinant mouse FGF-9.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-Fibroblast Growth Factor-9 antibody can be used in several immunochemical assays like dot blot and ELISA for the localization of FGF-9. It can also be used in microarray.

Physical form

Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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M Miyamoto et al.
Molecular and cellular biology, 13(7), 4251-4259 (1993-07-01)
Glia-activating factor (GAF) is a novel heparin-binding growth factor purified from the culture supernatant of a human glioma cell line. It shows a spectrum of activity slightly different from those of other known growth factors. We have isolated the cDNA

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