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Key Documents

LB58

98062325, human blood, Lymphoblastoid

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About This Item

UNSPSC Code:
41106514

product name

LB58, 98062325

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human lymphoblastoid, Ataxia telangiectasia, EBV-transformed

Cell Line Description

Established by EBV transformation of mononuclear cells from the peripheral blood of a 13 year old female with Ataxia telangiectasia (AT) complementation group C. AT is a rare autosomal recessive disorder. Lymphocytes have been shown to be hypersensitive to cytotoxic and clastogenic effects of gamma and X rays. T cells from the same original sample have been used for studies of mutant frequency at the hprt locus, with cells being described as Donor 58 or Patient 2 (see Lancet 1985). Fibroblasts derived from the same individual were reported to have RNA and genomic DNA sequence changes (del, 9nt).

Application

hprt mutation studies, toxicity assays

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.

Other Notes

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Certificates of Analysis (COA)

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