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C1805

Sigma-Aldrich

Anti-CD4 antibody, Mouse monoclonal

clone Q4120, purified from hybridoma cell culture

Synonym(s):

Anti-CD4 antibody, Mouse monoclonal

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Q4120, monoclonal

form

buffered aqueous solution

mol wt

59 kDa

species reactivity

human

technique(s)

flow cytometry: 5 μL using 1 × 106 cells

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

General description

Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. Cluster of differentiation 4 (CD4) human cell surface glycoprotein (59kDa) belongs to the immunoglobulin superfamily. It is expressed on the helper/inducer T cell subset, which is found on the majority of peripheral blood neutrophils, most cortical and mature medullary thymocytes, microglial cells, dendritic cells and on some malignancies of T cell origin.

Specificity

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Application

Monoclonal Anti-CD4 antibody produced in mouse has been used in indirect immunofluorescent staining.

Biochem/physiol Actions

Cluster of differentiation 4 (CD4) molecule binds to the major histocompatibility complex (MHC) class II molecules during the interaction of CD4+ T cells with antigen presenting cells or with target cells. It also serves as a high affinity cellular receptor for the GP 120 envelope glycoprotein of the human immunodeficiency virus (HIV-1, HIV-2). The CD4 molecule is involved in the adhesion of T-lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins. Lower levels of CD4 have been detected in monocytes, macrophages and granulocytes.

Target description

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W Popik et al.
Journal of virology, 72(8), 6406-6413 (1998-07-11)
We have previously shown that binding of human immunodeficiency virus type 1 (HIV-1) virions to CD4 receptors stimulates association of Lck with Raf-1 and results in the activation of Raf-1 kinase in a Ras-independent manner. In the present study, we
Engineered single human CD4 domains as potent HIV-1 inhibitors and components of vaccine immunogens
Chen W, et al.
Journal of virology, 85(18), 9395-9405 (2011)
Jason A LaBonte et al.
The Journal of experimental medicine, 196(4), 431-445 (2002-08-21)
HIV-1 naturally infects chimpanzees and humans, but does not infect Old World monkeys because of replication blocks that occur after virus entry into the cell. To understand the species-specific restrictions operating on HIV-1 infection, the ability of HIV-1 to infect
Expression of CD4 on human peripheral blood neutrophils
Biswas P, et al.
Blood, 101(11), 4452-4456 (2003)
Down regulation of CD4 expression following isolation and culture of human monocytes
Graziani-Bowering GM and Filion LG
Clinical and Diagnostic Laboratory Immunology, 7(2), 182-191 (2000)

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