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Sigma-Aldrich

Triethylammonium acetate buffer

volatile buffer, ~1.0 M in H2O

Synonym(s):

Triethylammonium acetate buffer, Buffer solution 1 M pH 7.0 (volatile)

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About This Item

UNSPSC Code:
12161700
PubChem Substance ID:
24889353
NACRES:
NA.25

Quality Level

200

Assay

0.95—1.05 mol

form

liquid

shelf life

limited shelf life, expiry date on the label

storage condition

dry at room temperature

concentration

1 M
~1.0 M in H2O

technique(s)

electrophoresis: suitable

color

colorless

refractive index

n20/D 1.357

pH

7.0

 

6.1

density

1.002 g/mL at 20 °C

suitability

suitable for chromatography
suitable for protein modification
suitable for separation of small nucleic acid fragments

application(s)

detection
diagnostic assay manufacturing
life science and biopharma
sample preparation

storage temp.

2-8°C

InChI

1S/C6H15N.C2H4O2/c1-4-7(5-2)6-3;1-2(3)4/h4-6H2,1-3H3;1H3,(H,3,4)

InChI key

AVBGNFCMKJOFIN-UHFFFAOYSA-N

General description

Triethylammonium acetate, also known as TEAA, is a volatile buffer widely utilized as a buffer in various biochemical and biological applications, particularly in peptide and proteomics research. It is a common choice for ion-pairing reagent in the chromatographic separation of oligonucleotides and plays a key role in buffer solutions for oligonucleotide synthesis and purification.

Application

Triethylammonium acetate has been used:
  • as a buffer in proteomics studies
  • as a component of the mobile phase to separate nucleotide sugars
  • as a buffer for the separation of glycopeptides in proteomics research

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Ready available solution reduce the need for preparation time

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9) (2013)
Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO)
Yang W et al.
Molecular Systems Biology, 14, e8486-e8486 (2018)
Weiming Yang et al.
Nature protocols, 15(8), 2589-2610 (2020-07-19)
Protein glycosylation is one of the most common protein modifications. A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond. O-GalNAcylation is thought to play
Sialic acid biosynthesis pathway blockade disturbs neuronal network formation in human iPSC-derived excitatory neurons
Mijdam R et al.
Journal of Neurochemistry, 167, 76-89 (2023)
Johannes Zander et al.
Infection and immunity, 76(4), 1333-1339 (2007-12-28)
Thymidine-dependent small-colony variants (SCVs) of Staphylococcus aureus are frequently associated with persistent and recurrent infections in cystic fibrosis patients. The phenotypic appearance of S. aureus SCVs or normal-colony variants (NCVs) is postulated to be affected by the intracellular amount of

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Analysis of Oligonucleotide Standard 6 Mix by Liquid Chromatography-UV

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

Analysis of Oligonucleotide Standard 6 Mix by Liquid Chromatography-UV

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

Analysis of Oligonucleotide Standard 6 Mix by Liquid Chromatography-UV

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

Analysis of Oligonucleotide Standard 6 Mix by Liquid Chromatography-UV

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

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