Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
12007C
Fetal Bovine Serum
Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture
Synonym(s):
Fetal Calf Sera, Fetal Calf Serum, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FBS, FCS, sera, serum
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About This Item
biological source:
bovine fetus
origin:
Australia origin
sterility:
γ-irradiated
sterile-filtered
sterile-filtered
shipped in:
dry ice
Pricing and availability is not currently available.
Recommended Products
biological source
bovine fetus
Quality Level
sterility
γ-irradiated
sterile-filtered
product line
FBS Premier
composition
Hemoglobin, ≤25 mg/dL
origin
Australia origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
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Application
FBS sourced from Australia is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Australia carries the best possible rating in relation to Geographical Bovine Spongiform Encephalopathy (BSE) Risk (GBR) as designated by the Scientific Steering Committee on the Geographical Risk of Bovine Spongiform Encephalopathy. The classification for Australia is GBR Level I.
Preparation Note
Gamma irradiation by SER-TAIN™ process (25-35kGy).
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Footnote
(Formerly product number F3297)
Legal Information
SER-TAIN is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Protocols
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Related Content
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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