MABN2431

Anti-phospho-NFL (Ser473) Antibody, clone 4F8

clone 4F8, from mouse

• Synonym(s): Neurofilament light polypeptide, 68 kDa neurofilament protein, Neurofilament triplet L protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4F8, monoclonal
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_006149
• UniProt accession no.: P07196
• target post-translational modification: phosphorylation (pSer473)
• Gene Information: human ... NEFL(4747)

Description

General description

Neurofilament light polypeptide (UniProt: P07196; also known as NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein) is encoded by the NEFL (also known as NF68, NFL) gene (Gene ID: 4747) in human. Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and in adrenal and extra-adrenal pheochromocytomas. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Mutations in NEFL gene can lead to Charcot-Marie-Tooth disease 1F and 2E disease that is characterized by demyelinating and progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms.

Specificity

Clone 4F8 specifically detects Neurofilament light polypeptide phosphorylated on Serine 473 in human and murine cells.

Immunogen

KLH-conjugted linear peptide corresponding to 15 amino acids surrounding phosphorylated serine 473. Cystine was added for conjugation to KLH.

Application

Anti-phospho-NFL (Ser473), clone 4F8, Cat. No. MABN2431, is a highly specific mouse monoclonal antibody that targets Neurofilament light polypeptide phosphorylated on Ser473 and has been tested for use in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected phospho-NFL (Ser473) in Immunohistochemistry applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Western Blotting Analysis: A representative lot detected phospho-NFL (Ser473) in Western Blotting applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-NFL (Ser473) in mouse brain tissue lysate.

Target description

~70 kDa observed; 61.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.