MABD76

Anti-Oct-4 Antibody, clone 9B7

clone 9B7, from mouse

• Synonym(s): POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9B7, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): human (immunogen homology)
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002692
• UniProt accession no.: Q01860
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POU5F1(5460)

Description

General description

The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Immunogen

Recombinant protein corresponding to human Oct-4.

Application

Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.

Target description

~39 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse embryonic stem cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable