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227040

Sigma-Aldrich

Anti-Cholera Toxin, B-Subunit Goat pAb

lyophilized, Calbiochem®

Synonym(s):

Anti-Cholera Antibody, Cholera Toxin Detection, Goat Anti-Cholera Toxin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

goat

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Goat polyclonal antibody supplied as lyophilized undiluted serum. Recognizes cholera toxin B-subunit.
Recognizes the B-subunit of cholera toxin.
This Anti-Cholera Toxin, B-Subunit Goat pAb is validated for use in Immunoblotting, Frozen Sections, Immunocytochemistry, ELISA for the detection of Cholera Toxin, B-Subunit.

Immunogen

Vibrio cholerae
non-denatured Cholera Toxin, B-subunit

Application

Immunoblotting (see application references)

Frozen Sections (1:4000; see application references)

Immunocytochemistry (1:200; see application references)

ELISA (1:10,000; see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute in 100 µl dH₂O. Further dilute with aqueous buffers, such as PBS.

Other Notes

Antibody should be titrated for optimal results in individual systems.
Vadolas, J., et al. 1995. Eur. J. Immunol. 25, 969.
Craig, J.P. 1971. Microbial Toxins 2A, 189.

Selected Citations
Peters, I., et al. 2009. Biochim. Biophys. Acta1788, 964.
Balasubramanian, N., et al. 2007. Nature Cell Biology9, 1381.
Nemchinov, L.G., Et al. 2000. Arch. Virol.145, 2557.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

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J Müthing et al.
Glycoconjugate journal, 14(1), 19-28 (1997-01-01)
In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay
Latoya L Allen et al.
Respiratory physiology & neurobiology, 261, 15-23 (2018-12-28)
Spinal chloride-dependent synaptic inhibition is critical in regulating breathing and requires neuronal chloride gradients established by cation-chloride cotransporters Na+-K+-2Cl- (NKCC1) and K+-Cl- (KCC2). Spinal transection disrupts NKCC1/KCC2 balance, diminishing chloride gradients in neurons below injury, contributing to spasticity and chronic
Sara Letzner et al.
The European journal of neuroscience, 52(6), 3561-3571 (2020-05-10)
Left-right differences in the structural and functional organization of the brain are widespread in the animal kingdom and develop in close gene-environment interactions. The visual system of birds like chicks and pigeons exemplifies how sensory experience shapes lateralized visual processing.
Annabel Guichard et al.
Cell host & microbe, 14(3), 294-305 (2013-09-17)
Cholera toxin (CT), a virulence factor elaborated by Vibrio cholerae, is sufficient to induce the severe diarrhea characteristic of cholera. The enzymatic moiety of CT (CtxA) increases cAMP synthesis in intestinal epithelial cells, leading to chloride ion (Cl(-)) efflux through
Mia N Kelly et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 318(6), R1058-R1067 (2020-04-30)
Circadian rhythms are endogenous and entrainable daily patterns of physiology and behavior. Molecular mechanisms underlie circadian rhythms, characterized by an ~24-h pattern of gene expression of core clock genes. Although it has long been known that breathing exhibits circadian rhythms

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