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G005709

LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase: [A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B)
flow rate: 0.5 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 100-1000
injection: 0.5 μL
sample: 200 μg/L in 25:75, water: acetonitrile

suitability

application for HPLC

application(s)

forensics and toxicology

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    EDDP perchlorate solution, 1.0 mg/mL in methanol (as pyrolinium), ampule of 1 mL, certified reference material, Cerilliant®
    (±)-Methadone solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    EMDP hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

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