ZRB2964

Anti-CXCR2/IL-8RB Antibody, clone 2D18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B (IL-8R B), IL-8 receptor type 2
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2D18, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 41 kDa; observed mol wt ~51 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal extracellular domain
• Protein ID accession no.: NP_001161770
• UniProt accession no.: P25025
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CXCR2(3579)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2D18 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CXCR2/IL-8RB. It targets an epitope within 25 amino acids from the N-terminal extracellular domain of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal extracellular domain of human CXCR2/IL-8RB.

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CXCR2/IL-8RB in HepG2 cell lysate.

Tested Applications

Affinity Binding Assay: The binding affinity of this antibody to CXCR2/IL-8RB peptide was at least five thousand-fold (5,000X) higher than that obtained with the non-specific control peptide.

Flow Cytometry Analysis: 1 µg from a representative lot detected CXCR2/IL-8RB in one million HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CXCR2/IL-8RB in HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CXCR2/IL-8RB in human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

C-X-C chemokine receptor type 2 (UniProt: P25025; also known as CD182, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B (IL-8R B), IL-8 receptor type 2) is encoded by the CXCR2C (also known as IL8RB) gene (Gene ID: 3579) in human. CXCR2/IL-8RB belongs to the G-protein coupled receptor 1 family. It is a membrane protein composed of seven transmembrane domains. It has a conserved DRY motif involved in G protein coupling. CXCR2 also contains N-linked glycosylation sites that can affect receptor trafficking and function. It primarily acts as a receptor for chemokines. Upon ligand binding, CXCR2/IL-8RB activates intracellular signaling pathways, including G protein-mediated signaling cascades and recruitment of β-arrestins. These signaling events regulate various cellular processes such as chemotaxis, neutrophil activation, angiogenesis, and immune cell recruitment. CXCR2/IL-8RB is expressed in a wide range of immune cells, including neutrophils, monocytes, T cells, and natural killer cells. It is also found in non-immune cells, such as endothelial cells and epithelial cells. The expression level of CXCR2 is predominantly induced during inflammation and infection. CXCR2 is primarily localized on the cell surface, where it functions as a membrane-bound receptor. It can also undergo internalization upon ligand binding, leading to receptor desensitization and subsequent downstream signaling events. Due to its role in immune regulation and inflammation, it has been associated with inflammatory conditions such as chronic obstructive pulmonary disease (COPD), asthma, rheumatoid arthritis, and inflammatory bowel disease. Additionally, CXCR2 has been implicated in cancer progression and metastasis by promoting angiogenesis, tumor growth, and metastatic cell migration. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable