U0508
Monoclonal Anti-Ubiquitin antibody produced in mouse
clone 6C1, ascites fluid
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About This Item
conjugate:
unconjugated
application:
ARR
ELISA (i)
WB
ELISA (i)
WB
clone:
6C1, monoclonal
species reactivity:
human, rat, mouse, bovine
citations:
37
technique(s):
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
microarray: suitable
western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6C1, monoclonal
contains
15 mM sodium azide
species reactivity
human, rat, mouse, bovine
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UBB(7314) , UBC(7316)
mouse ... Ubb(22187) , Ubc(22190)
rat ... Ubb(192255) , Ubc(50522)
General description
Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.
Immunogen
purified bovine ubiquitin conjugated to KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby
Andrej Susor et al.
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In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis
Mandy H Y Lam et al.
PloS one, 8(9), e75372-e75372 (2013-09-27)
The yeast HECT-family E3 ubiquitin ligase Rsp5 has been implicated in diverse cell functions. Previously, we and others [1], [2] reported the physical and functional interaction of Rsp5 with the deubiquitinating enzyme Ubp2, and the ubiquitin associated (UBA) domain-containing cofactor
Alexander Espinosa et al.
The Journal of biological chemistry, 286(42), 36478-36491 (2011-08-25)
Ro52 (TRIM21) is an E3 ligase of the tripartite motif family that negatively regulates proinflammatory cytokine production by ubiquitinating transcription factors of the interferon regulatory factor family. Autoantibodies to Ro52 are present in patients with lupus and Sjögren's syndrome, but
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